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Home » Archive » 2008

TDK conference 2008

Quantitative, subgenomial mRNA detecting to separate FIP and feline enteral corona virus
Kosztolányi Orsolya - year 4
Department of Microbiology and Infectious Disease
Supervisor: Dr. Ákos Hornyák

Abstract:

Feline infectious peritonitis (FIP) is one of the most dreadful diseases for the cat owners. Once the clinical signs have appeared the sick animal can not be cured, so establishing a correct FIP diagnosis is of high importance. The authors developed a novel quantitative method for the FIP diagnosis which combines the quantification of the virus and its M gene sg-mRNA detection. In order to facilitate a broad range detection of cat CoV PriProET system was applied, which is published to be less sensitive to the nucleotide substitutions on the target area. The sg-QPCR method sensitivity was found below 10 copies, the specificity of the test was proven by the successful detection of nine different FCoV strains. Faecal samples of healthy young cats, organ samples of perished animals having suffered in FIP and cat leucocytes from uncertain cases were involved in the examinations. The quantitative sg-mRNA detection method revealed a 10-50000 times more virus M gene quantity in the checking materials of FIP cases comparing to that of the enteric CoV variants present in the faeces of normal healthy cats, proving the applicability of this test for the FIP diagnosis.



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