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Home » Archive » 2011

TDK conference 2011

Retrospective analysis of gastric biopsy samples taken with endoscope from dogs
Hőnich Eszter - graduating student
Szent István University Faculty of Veterinary Science Department and Clinic of Internal Medicine
Supervisor: Roland Psáder DVM

Abstract:

The histopathological examination of gastric biopsies provided by gastric endoscopy is necessary for the early identification, and successful treatment of gastric inflammations and tumors.

Our goal was retrospective processing of the gastric biopsy samples taken from canine patients coming for gastroscopy, in accordance with the new international standard (Day et al.: Histopathological standards for the diagnosis of gastrointestinal inflammation in endoscopic biopsy samples from the dog and cat: a report from the WSAVA Gastrointestinal Standardization Group. J. Comp. Pathol. 2008). By evaluating the processed samples, we examined if there was a correlation between the age and gender of the patients, the degree of the histiopathologic lesions and the intensity if the treatment necessary.

Materials and method: We took 3-3 samples from the three anatomical regions (fundus, corpus, antrum) of the stomach using a biopsy forceps during the gastroscopic examination, which was carried out in narcosis. From the january of 2007 to this day, we examined 66 samples from 60 dogs (age ranging between 0,5-13,5 years, 33 male dogs, 27 bitches). The histopathologic processing of the samples (Hematoxilin-eosin stain, modified Giemsa stain, Warthin-Starry stain when needed) took place in the Department of Pathology and Forensic Veterinary Medicine. During the evaulation of the histopathologic samples, we marked the degree of lymphocitic-plasmacellular and eosinophilic infiltrations, lymphoid follicular hyperlpasia, and GHLO infections on a scale of zero to three (not present, slight, medium, severe). The stages of treatment were 1: symptomaptic treatment, 2: simple diet, 3: gluten free diet with the addition of a new protein source, 4: simple antibiotic treatment, 5: Helicobacterium eradication, 6: prednisolon. We evaluated the results with statistic methods.

Conclusion: The GHLO infection, the degree of histiopathological lesions, and the intensity of treatment do not show clear relations with each other, which statement is in accordance with the data in the literature. It is surprising, that the degree of the histopathological lesions do not correlate with the intensty of the treatment used, so the immunhistochemical examination of the same samples for possible markers and prognostic factors is our goal in the future.



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