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Home » Archive » 2013

TDK conference 2013

Diversity of fungal spores in floral and honeydew honeys
Mura-Mészáros Anna - year 3
NIEH, SzIU, Faculty of Veterinary Science, Department of Botany
Supervisors: Donát Magyar dr., Irén Siller dr.

Abstract:

Nowadays, quality control is an important issue in food safety. Little research has been done so far in identifying the fungal constituents of honeys. However, carrying out a qualitative analysis of them may play an important role in determining the geographical and botanical origin of the product, mainly of honeydew honeys which could be characterized by the quantity of fungal elements. Qualitative analysis showed that there is some difference in the botanical origin of honeydew honeys collected in Greece. The aim of this study was to add new records to and analyse the database of the spore composition of the honeys in a Hungarian collection. New data of 10 honey samples was added to the database. A total of 92 samples was analysed from three continents (Europe, Africa and Central-America) and 20 countries (most of them from Italy, Greece and Hungary). The samples were prepared and analysed microscopically according to a standard melissopalynological method. A total of 222 different types of fungal spores were present int he samples, 59 of them were only recorded once. 101 and 83 taxa were identified at genus or species level, respectively. More than fifty percent of the samples contained spores belonging to the species of Alternaria (86,96%), Epicoccum (81,52%), Cladosporium (61,96%), Stemphylium (57,61%), Periconia (54,35%) and Pucciniceae (54,35%), Agaricomycetes (53,26%) and Melampsoridium / Cronartium / Melampsora spp. (52,17%). The number of spore types was higher in honeydew honeys (avg. 30.69 ± 14,98) than in floral honeys (avg. 11,61 ± 5,69). Nonparametric method showed that the two groups differ significantly (p=1,22e-08). Principal component analysis was performed to find possible indicator taxa by the groups of honeys formed based on qualitative data. Our results on spore composition of honeys could be used to find indicator species of the different geographical and botanical origins.



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