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Home » Archive » 2013

TDK conference 2013

Serotypes of Actinobacillus pleuropneumoniae strains in Hungary
Horváth Pálma - year 5
SzIU, Faculty of Veterinary Science, Department of Microbiology and Infectious Diseases
Supervisor: Sárközi Rita DVM

Abstract:

Actinobacillus pleuropneumoniae is a Gram-negative, facultative anaerobic, fastidious bacterium which causes respiratory disease in swine throughout the world. Haemorrhagic-necrotic pneumonia and fibrinous pleuritis can be seen in growing pigs. The bacterium is facultative pathogenic, it is present on the mucous membrane of the respiratory tract of swine and can cause disease in case of different predisposing factors.

On the basis of NAD requirement for growth A. pleuropneumoniae biotype 1 (NAD-dependent) and biotype 2 (NAD-independent) can be distinguished. After combining the previous typing systems 15 serotypes can be differentiated.

The aim of the study was to collect data on the frequency of the different serotypes and compare the results of serotyping with the indirect haemagglutination test and PCR test detecting toxin genes.

Sixty biotype 1 (NAD-dependent) A. pleuropneumoniae strains from the strain collection of the Department of Microbiology and 30 biotype 1 A. pleuropneumoniae strains isolated from pneumonic lung samples collected in two slaughterhouses were examined. Primary and secondary cultural, morphological and biochemical features of the bacterial isolates were tested.

The strains were serotyped on the basis of surface antigens with indirect haemagglutination test. As a result of serotyping 46 strains proved to be of serotype 2, 6 strains were serotypes 8, 12 strains were serotypes 9, 21 strains were serotype 12 and 5 strains could not be serotyped with hyperimmune sera. Since toxin production is characteristic to the different A. pleuropneumoniae serotypes the multistep polymerase chain reaction (PCR) technique can be used for serotyping. Using this PCR test the A. pleuropneumoniae strains could be allocated to the appropriate serotype specific toxin groups.



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