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Home » Archive » 2013

TDK conference 2013

Anti-inflammatory effect of probiotics and sodium-n-butyrate in the intestinal epithelium
Lencse Zsófia - year 5
SzIU, Faculty of Veterinary Science, Department of Pharmacology and Toxicology
Supervisors: Orsolya Farkas, Orsolya Palócz

Abstract:

Probiotics have received increasing attention in recent years as supporters of the metabolism and enhancers of the immune response. European Union (EU) banned the use of antibiotics as growth promoters in swine and livestock production on 1 January 2006. For this reason, there is a growing interest to replace the use of antibiotics by different natural alternatives, such as probiotics. Our aim is to investigate the anti-inflammatory activity of spent culture supernatant of certain probiotic microbes (e.g. Lactobacilli) and their single microbial products (sodium-n-butyrate).

IPEC-J2 cells were seeded onto collagen-coated polyester membrane inserts. Transepithelial electrical resistance measurement of cell monolayer was performed in the growth period and during the experiments. Inflammation was evoked by bacterial lipopolysaccharid (LPS) in the epithelial cells. IL-8 and TNF-α were assayed as markers of the degree of inflammation. The expression of these cytokines was detected by qPCR at the level of transcription (in IPEC-J2 cells) and by ELISA at the level of translation (in the basolateral culture medium). The studied bacterial strains were the following: Lactobacillus plantarum 2142 (Lp 2142), Lactobacillus plantarum 299v (Lp 299v) and Lactobacillus casei Shirota (Lc. Shirota).

The TNF-α and IL-8 relative gene expression in IPEC-J2 cells were increased significantly, after treatment cells with 1 μg/ml, or 10 μg/ml LPS, respectively. 10 μg/ml LPS was applied when anti-inflammatory effect of probiotics and butyrate was tested. These studies showed, that Lp 2142 (13,3 v/v%) spent culture supernatant suppressed the IL-8 and TNF-α gene expression, whereas Lp 299v and Lc Shirota did not caused the same positive effect. After treatment with butyrate (2 mM) and LPS in same time, the IL-8 and TNF-α gene expressions were both reduced.

It can be concluded, that the SCS of Lp 2142 and sodium-n-butyrate have anti-inflammatory effects. Our further aim is to test impact of other bacteria (e.g. Bacillus, Bifidobacterium) in reducing inflammation and follows more parameters (IL-6 gene expression and concentration, TLR-4 receptor) in our model system.



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