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TDK conference 2013

Microflora in focus: a retrospective analysis of skin scraping and ear samples
Varga Andrea - year 6
SzIU, Faculty of Veterinary Science, Deparmtnet and Clinic of Internal Medicine
Supervisors: Dr. Tarpataki Noémi, Dr. Lajos Zoltán


Due to the increase in number of bacterial skin and ear disease cases and with the expanding antibiotic-resistance, it is important to study the current microbiological status of our companion animals.

The data used in this study were microbiological analysis results (conducted in a widely acknowledged and standardized procedure by DuoBakt Microbiological Lab) of the skin scraping and ear samples of the patients examined between January 1, 2008 and August 31, 2013 at the dermatology consultation of Dept. and Clinic of Internal Medicine of SZIE ÁOTK. Data were collected from the software of Doki for Vets of the SZIE-ÁOTK Small Animal Clinic and the DuoBakt Microbiological Lab’s own utility. Lab. Statistical analysis was made by MySQL database management system and Microsoft Excel 12.0 programs.

As a basis for this study 750 positive (for bacterium and/or yeast) skin scraping (686 dog, 64 cat) and 139 positive ear samples (131 dog and 8 cat) were examined. Among the pathogens found in these dogs, a significant 667 times Staphylococcus pseudintermedius (S.p.) and 42 times ß-haemolitic Streptococcus (ß-haem. Str.) were cultivated, also 7 and 7 times Staphylococcus aureus (S.a.) and Pseudomonas aeruginosa ( were found, respectively. Regarding yeasts, Malassezia pachydermatis (M.p.) was also found in 8 cases. Among the cat’s skin scraping samples 36 S.p. and 24 S.a. were found. Out of 131 positive dogs’ ear samples in 76 cases S.p. also in 40 cases and in 33 cases ß-haem. Str. were confirmed. The presence of yeast was also quite remarkable: M.p. was detected in 37 ear samples while Candida spp. in only two cases.

The antimicrobial susceptibility of the samples was also included in this study. The S.p. bacteria which is the most common cause of dog skin inflammation has shown susceptibility to the amoxicillin-clavulanic acid (AMC) in 96,10% of the cases, while the percentage of resistance was 27,44% to clindamycin (CLI). The ß-haem. Str. cultivated from skin scrapes was 97,62% susceptible to AMC, however 50,00% resistant to gentamycin (GEN), and regarding ciprofloxacin (CIP) and tobramycin (TOB) was 100,00% effective, while showed 28,57% resistance to GEN. Referring to ear samples: S.p. was 97,37% susceptible to AMC, P.ea. was 95.00% susceptible to TOB. S.p. had shown 23,68% resistance to CLI, while ß-haem. Str. was 96.97% susceptible to the same antibiotic. In the case of GEN: S.p was 25,00%, ß-haem. Str. was 63,64% and was 22,50% resistant. Out of the cat’s skin scrape samples, S.p. has shown 100,00% susceptibility, while S.a. had shown 87,50% resistance to PolymyxinB. The susceptibility to AMC was in 94,59% of the cases of S.p. and 100,00% for S.a. Data was also examined using several other viewpoints and the results can be read in this work.

In conclusion, the relevance of microbial analysis must be emphasized against the more common but often old-fashioned empirical treatment in the small animal praxis.

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