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Home » Archive » 2015

TDK conference 2015

Trout furunculosis caused by Aeromonas salmonicida subsp. salmonicida: treatment optimalization in practice
Zsigmond Gergely Domonkos - year 4
HAS CAR, Institute for Veterinary Medical Research, Fish Parasitology Research Team; SzIU, Faculty of Veterinary Science, Department of Pathology
Supervisors: Dr. Edit Eszterbauer, Ferenc Baska DVM

Abstract:

Our research focused on the practicable treatment methods of furunculosis caused by the bacterium Aeromonas salmonicida subsp. salmonicida. This pathogen causes large-scale damages in trout farms, due to both the treatment and decontamination expenses, and the loss of production.

The most widespread treatment of the disease is the use of various antibiotics. The environmental impact of the treatment and the risk of emergence of resistant strains are long-known problems.

The main purpose of our research was to elaborate an antibiotic treatment protocol, which is easily applicable in practice, and besides the minimization of the environmental impact, it guarantees the delivery of the necessary dose of antibiotics.

For this purpose, first we isolated strains from fish suffering from furunculosis, and identified them. Based on their resistogram, we chose enrofloxacin (ENR) to accomplish a treatment experiment. In the 10-day experiment at the Lillafüred Trout Farm, we developed 3 groups with different treatment methods: intraperitoneal injection (IP), a medicated diet with ENR-coated granules (BEV), and a medicated diet with homogenized and reformatted granules containing ENR (HOM). The fourth group was the control. At the end of the experiment, we took samples of muscle tissues, in which we measured the ENR concentration with an ELISA-based method.

Our results show, that whereas the IP treatment eventuated a significantly higher drug concentration in the tissue, we could not detect significant difference between the two medicated diets. But it appears from the standard deviation values that the IP method grants a more accurate delivery of the drug. In addition, there was a problem in the course of the experiment: the fish did not consume the necessary amount of fish pellet, supposedly because of the medicine’s extremely bitter taste.



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