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TDK conference 2015Bozi Szilvia - year 5 SzIU, Faculty of Veterinary Science, Institute for Animal Breeding, Nutrition and Laboratory Animal Science Supervisors: Gabriella Korsós DVM, Dr. Sándor György Fekete The noise is a general stressor both for human and laboratory animals. As a consequence (patho)phisiological changes can be expected. To get accoustomed animals to abrupt noises (e.g. thunderstorm) holds a great significance in the scientific research and also in the practical life. As a model animal twelve male ChR (W1) barrier raised SPF rats were used. The animals were allocated in two separate rooms. The first group was exposed (70 dB) during twelve days from 8 a.m. until 8 p.m. a mixture of different technical and natural noises (in the following "N" group). The control group (in the following "C") lived in a quiet isolated room. Three open-field (OF) investigation have been made; first the basic (B) behaviour patterns were studied. After that the noise mixture (N1, N2) was used (80-90 dB). After pentobarbital oversleeping blood samples were taken for chorticosterone analysis; and the important organs and tissues were sent for histopathological study. During the necropsy the weight of testis, spleen, suprarenal glands and thymus was measured. No differencies were found in the organ weight and chorticosterone level. The histopatological investigation has no revealed any morphological stress signs. During the noisy OF the noise-treated groups spent significantly less time with crouching, much more time with sniffing while rearing and sniffing while running. Compared to the basal ethogram, both during N1 and N2 treatement, the number of crossing the center decreased while the number of rearing was increased but only during N2 significantly. During N1 the rats spent significantly more time with sniffing standing still. The rats spent significantly more time running during N2. During both N1 and N2 the animals spent significantly more time with crouching and less time with sniffing while rearing and sniffing while running. Giving the fact, the physiological and pathological parameters did not change significantly, one can state that the noise exposure cannot consider a substantial stressor. To sum up, the noise-treated group crouched less, the inquisitory behaviour increased (more sniffing, moving) during the OF tests. The results show that in certain experiment the noise treatement may counteract the failure of the acoustic environment, improving the animal wellfare. List of lectures |