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TDK conference 2015

Accuracy of diagnosing double corpora lutea and twin pregnancy by measuring serum progesterone and bovine pregnancy-associated glycoprotein-1 in the first trimester of gestation in dairy cows
Kiss Bence - year 6
SzIU, Faculty of Veterinary Science, Department and Clinic for Production Animals
Supervisor: Zoltán Szelényi DVM


In our study we evaluated the serum progesterone (P4) and the bovine pregnancy-associated glycoprotein-1 (bPAG-1) concentrations at different stages of pregnancy (Days 29-42, Days 57-71, Days85-98, Days 113-126) in 84 Holstein-friesian dairy cattle, with the aim to disseminate cattle having 1 or 2 corpora lutea and twin and singleton pregnancies.

We divided the animals into 3 groups based on the pregnancy ultrasound examination: 2 embryo and 2 corpora lutea (group 1), 2 embryo and 1 corpus luteum (group 2), 1 embryo and 1 corpus luteum (control group). All animals calved according to the early pregnancy diagnosis. Blood sample was taken from each animal 4 times during the different stages of pregnancy. We measured the P4 concentration in the sample with validated, solid phase 125I RIA method and to measure the bPAG-1 concentration, we used polyclonal antiserum. Statistical analysis was performed with Minitab 16 software.

Analyzing the P4 and bPAG-1 concentrations, we found that there was a significant difference in bPAG-1 concentrations between the groups and the times, but the P4 concentration showeddifference between only the groups. To find the connection between the P4 concentration and corpus luteum number we redistributed the animals based on the number of the corpora lutea. There was a significant difference between P4 concentration only in the samples that were taken at the second time point. To find the connection between bPAG-1 concentration and the number of embryo/fetus, we redistributed the animals based on the fetus number. There was a significant difference in bPAG-1 concentration between the samples at the 4 different time points. Evaluating the ROC (receiver operating characteristic) diagram resulted accurate cut-off point regarding to the serum P4 concentration based on the samples taken at the second time point with the highest sensitivity and specificity. Regarding to the bPAG-1 concentrations the area under the ROC diagram was acceptable.

Based on our results in pregnant animals the number of the corpora lutea didn`t show effect to the serum P4 concentrations, although the raise of the P4 concentration indicates the pregnancy precisely. Unlike the P4, to measure the bPAG-1 concentration is useful to distinguish the singleton pregnancies from twins, accurate diagnose can be performed in latter stages of pregnancy. However, the definitive diagnosis of pregnancy by measuring serum proteins cannot be evaluated because of the low specificity of the samples.

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