TDK conference 2016

The Sangrovit and Immunofort are commonly used drinking water additives for several species of animals. These products contain numerous substances that facilitate the health of the chicken stock, because they have antibacterial effect and they can slightly decrease the pH of the crop and coecum, and they have beneficial effect on bacterial enzyme activity.

During the treatment of the animals against some common diseases, such as bacterial infection and coccidiosis, there are some factors that we must take into consideration. One of these factors is the effect of cytochrome enzymes. These enzymes have an important role in the metabolization of the xenobiotics, such as medicines and other chemical substances.

The question arises, what effect these feed additives have on the cytochrome enzymes, mainly on their expression and the activity.

In our experiment, we treated broiler chickens divided into five groups to determine these effects. We had a group that got low dose Sangrovit (5 mg/kg bw.), high dose Sangrovit (50 mg/kg bw.), low dose Immunofort (0.1 ml/l) and high dose Immunofort (1 ml/l) and we had a control group. The high dose Sangrovit and low dose Immunofort treatment downregulated the CYP2H1 gene expression.. Both the high and low dose Immunofort treatment decreased the CYP1A1 gene expression level. The low dose Sangrovit and both doses of Immunofort increased the activity of the CYP1A1 enzyme.

The CYP2H subfamily has significance in the drug metabolism of birds, smaller proportion of the active substances decomposed on the CYP3A and CYP1A subfamilies. The additives applied in our study in none of the used concentrations altered considerably the gene expression and enzyme activity of the investigated isoenzymes. Therefore these substances safely applicable in chicken stocks directly before drug therapy, neither withdrawal time nor dose adjustment necessary before administration of the medication.