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Home » Archive » 2017

TDK conference 2017

Investigation on methicillin- resistant Staphylococcus aureus strains isolated from the Department and Clinic of Equine Medicine UVMB
Morvay Flóra - year 6
University of Veterinary Medicine, Department and Clinic of Food Animal Medicine, Department and Clinic of Equine Medicine
Supervisors: Dr. Ervin Albert, Dr. Zoltán Bakos

Abstract:

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) infection is currently a serious challenge to veterinary clinicians due to the widened antibiotic resistance spectrum of certain clonal lineages. At the Department and Clinic of Equine Medicine, University of Veterinary Medicine Budapest (DCEM UVMB) the first MRSA was isolated in 2011. After this, the number of methicillin-resistant isolates and related clinical cases increased year by year, raising the need for a meticulous investigation on the concerned isolates and related clinical documentation to declare and resolve the matter.

Detailed characterisation of the isolates were carried out by means of selected molecular biological methods (mecA PCR, spa-typing, MLST) and by antimicrobial susceptibility testing. Clinical data of related cases were collected to support better evaluation of microbiological findings.

Between July 2011 and May 2016 an overall of 40 Staphylococcus aureus strains originating from 36 horses were assigned to MRSA. The first isolate represents the sequence type ST398 and spa-type t011, respectively. This isolate was resistant to penicillins, cefoxitin, tetracycline, streptomycin, gentamicin, kanamycin and ciprofloxacin. This MRSA with the identical genotype and resistance profile was isolated on 21 more occasions from September 2013 to September 2014. Then 8 months passed without any new case, when a second outbreak started in May 2015. In this outbreak the first isolate still shared the same genotype but was additionally resistant to chloramphenicol and the next one from August 2015 showed resistance to rifampin, too. In this second outbreak from May 2015 to May 2016 the clone was isolated 18 times.

The methicillin-resistant S. aureus related cases at the DCEM were parts of a nosocomial infection caused by the same MRSA clone ST398-t011. Based on the results of our survey we have introduced routine screening of patients, monitoring changes in the antibiotic profile of MRSA isolates and strict hand hygiene regulations. These resulted in a significantly lowered incidence of MRSA isolation and related clinical disease.



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