TDK conference 2017

The objective of this study was to examine the influence of dietary organic selenium supplementation on the activity of alkaline phosphatase (ALP, E.C. 3.1.3.1) and concentration of zinc in tissues of chickens at the end of the fattening period and after 48-hours fasting. The research was done on the hybrid line of chickens, Ross 308. During first seven days chickens were fed with commercial diet, after which they were randomly allocated in two pens. The control group was fed with standard diet till the end of the fattening period, and to the experimental group (Selen+) was, the seventh day after hatching till the end of research, given 0.3 ppm organic selenium. Till the age of 42 days, both groups of chicken were given both food and water ad libitum. After that, they were exposed to 48-hour fasting period with access to water ad libitum. After the end of a fattening period and after 48-hour fasting, 10 chickens were randomly selected from Selen+ group, and 10 from the control group. Immediately after sacrificing the chickens, the samples of liver, kidney, small intestine, heart, and pectoral and thigh muscle tissue were taken for analysis. Alkaline phosphatase activity and concentration of zinc were determined in tissues. Dietary organic selenium supplementation decreased the ALP activity in the liver (p<0.05), and increased zinc concentration in liver (p<0.05), kidney (p<0.001), thigh muscle (p<0.05) at the end of the fattening period. After 48-hours food deprivation, organic selenium supplementation resulted in higher ALP activity in the small intestine (p<0.001), and lower activity in thigh muscle (p<0.05). The ALP activity in standard-fed chicken after 48-hour fasting was decreased in liver (p<0.001), kidney (p<0.01) and also in small intestine (p<0.01), along with the increase of zinc concentration in liver (p<0.05), kidney (p<0.001) and small intestine (p<0.01). Inorganic selenium supplemented the chicken decrease in ALP activity was measured in liver (p<0.01), kidney (p<0.01), and also in pectoral muscle (p<0.05). Simultaneously in the small intestine and in the heart, the ALP activity was increased (p<0.001 respectively p<0.05) after the 48-hour fasting period. The supplementation of organic selenium caused an increase in ALP activity only in the small intestine after a 48-hour fasting period, along with the increase of zinc concentration in liver, kidney and thigh muscle of chicken at the end of the fattening period, probably due to increased absorption from the intestine and excretion through the kidney. Since fasting leads to lower activity of ALP in organs and tissue studied, monitoring the level of ALP could be used as a marker for nutrition disorders in intensive chicken production.

Keywords: chicken, alkaline phosphatase, organic selenium, zinc, tissue, fasting