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Home » Archive » 2018

TDK conference 2018

Detection of fetal DNA from maternal plasma samples with PCR in horse
Vöröskői Petra Anna - year 5
University of Veterinary Medicine Budapest, Department Animal Breeding, Nutrition and Laboratory Animal Science
Supervisors: Dr. Vincze Boglárka, Dr. Zenke Petra

Abstract:

Sex determination has a high significance since the spreading of embryo manipulation in horse breeding. When biopsied embryos before implantation, such sex specific gene sequences can be isolated from DNA only stored in one cell, which only characterizes females or males. As known for a few years, a small amount of fetal DNA passes through the horses’ multilayer placenta during pregnancy, but this procedure is so new, that many questions about the extraction, replication and about the appropriate detection method sex specific genome sequences of the fetal DNA have not been cleared yet. Our research is based on the already published methods about DNA-cleaning, PCR protocol and applied primers, but we did not achieve certain specificity and sensitivity from the samples derived from different pregnancy periods, mainly from the last pregnancy period in order to determine the fetal sex. That is the reason why it was necessary to try a new method planned and developed in our laboratory, based on its result we could improve the efficiency of the procedure from more point of views.



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