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Home » Archive » 2018

TDK conference 2018

Comparative examination of detoxifying processes in wild boar and domestic pig
Kurucz Ádám - year 4
University of Veterinary Medicine Budapest, Department of Physiology and Biochemistry
Supervisors: Dr. Gábor Mátis, Dr. Kata Orbán

Abstract:

Drug-metabolizing cytochrome P450 (CYP) enzymes are highly involved in hepatic and intestinal detoxifying processes, and their function is of special importance in wild animals, directly exposed to environmental pollutants. Concerning hepatic CYPs, limited data are available regarding wild ruminants, while absolutely no information can be found about wild boar and with regard on the intestinal drug metabolism in any hunted wild animal species. In this study, we aimed to assess the specific activity of certain CYP enzymes playing key role in xenobiotic biotransformation in wild boar and domestic pig.

Liver and intestinal mucosa (from duodenum, jejunum, ileum and caecum) samples were freshly collected from 49 hunted wild boars and 15 more liver samples from wild boar foetuses in Western Hungary, while domestic pig samples (n=40) were gained from a slaughter house. The post-mitochondrial supernatant containing CYP enzymes was isolated after homogenization of tissue samples in phosphate buffer by a multi-step differential centrifugation. Specific activity of CYP1A2, CYP2C9 and CYP3A4 enyzmes was assessed by luminometric P450-Glo assays.

The activity of hepatic CYP1A2 enzymes was significantly (P=0.008), approximately 4-fold higher in wild boars than in domestic pigs. Similarly, the activity of CYP3A4 was found to be significantly (P<0.001), approximately 8-fold increased in the liver of wild boars when compared to those of domestic pigs. In contrast, hepatic CYP2C9 had a significantly (P<0.001), 50% lower activity in wild boars than in domestic pigs. . The activity of intestinal CYPs was under detection level in both species in all intestinal sections. The activity of foetal hepatic CYP2C9 was detectable in foetuses from the last trimester, but it was remarkably lower than in adult wild boars.

According to our results, great differences could be observed in hepatic drug-metabolizing CYP activities between wild boars and their domestic counterparts. The described isoenzyme-specific, species-related alterations might be explained with the different exposure of wild and domesticated animals to specific CYP modulators, taken up from the environment or with the diet. As the activity of CYPs in wild boars can be highly influenced by environmental pollutants, following further studies, CYP enzymes may be applied as ecotoxicological markers of common agricultural or industrial toxicants. Investigating CYP-related drug metabolism in wildlife species can clarify some possible toxicokinetic interactions, thus having huge importance in the production of safe game meat, being free of toxic residues.



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