Students' Research Circle    
 
 
2022
2021
2020
2019
» 2018
Call for papers
The conference
Veterinary Session
Veterinary Jury
Biology Session
Biology Jury
Sponsors
Awards-list
2017
2016
2015
2014
2013
2012
2011
2010
2009
2008
2007
2006
2005
2004
2003
2002
Home » Archive » 2018

TDK conference 2018

The usage of spectrophotometer in measuring boars semen concentration and the investigation of factors influencing it
Fülöp Dorottya - year 5
University of Veterinary Medicine Budapest, Department and Clinic of Food Animal Medicine
Supervisor: Dr. András Horváth

Abstract:

Main intention of the research is to examine the precision of the spectrophotometric measurements, the calibration of the method and how to use it. Usually farms neither have the time nor the opportunity to use microscopic examination of the sperm concentration, so the spectrophotometric can be used as an alternative for quicker, simpler, more suitable measuring method but less punctual. The purpose of our research is to calibrate the spectrophotometric measurements, to refine its accuracy and to compute a calculation method by using the Makler-chambers precisely counted concentrations. With a correctly counted concentration we can add more semen extender, therefore getting bigger portions with the right semen concentration, so the usage would be more economical for the farms. For the research 33 semen samples were taken from boars in two different farms. For every samples we made two eight-dilution lines: one diluted by semen extender liquid and one by 3% saline solution. We measured all diluted solution with spectrophotometer and then counted their concentration. While in the samples mixed with the semen extender the sperms were alive, on the other hand in the samples mixed with the 3% saline solution the sperms were killed. Then spectrophotometric concentration results were compared to the samples concentration in the Makler-chambers and searched for correlations between them. As a result, the following were obtained: all of the gold standard Makler-chambers concentration were compared to all spectrophotometric concentration and as a result for correlation coefficient we got 0,89. The highest correlation coefficient we got as a result was 0,70 diluted by semen extender on dilution 1:6, and the second highest coefficient was 0,69 on dilution 1:4. We got similar result when the diluent was 3% saline solution on dilution 1:6. There the correlation coefficient is 0,68.



List of lectures