TDK conference 2018

Main intention of the research is to examine the precision of the spectrophotometric measurements, the calibration of the method and how to use it. Usually farms neither have the time nor the opportunity to use microscopic examination of the sperm concentration, so the spectrophotometric can be used as an alternative for quicker, simpler, more suitable measuring method but less punctual. The purpose of our research is to calibrate the spectrophotometric measurements, to refine its accuracy and to compute a calculation method by using the Makler-chambers precisely counted concentrations. With a correctly counted concentration we can add more semen extender, therefore getting bigger portions with the right semen concentration, so the usage would be more economical for the farms. For the research 33 semen samples were taken from boars in two different farms. For every samples we made two eight-dilution lines: one diluted by semen extender liquid and one by 3% saline solution. We measured all diluted solution with spectrophotometer and then counted their concentration. While in the samples mixed with the semen extender the sperms were alive, on the other hand in the samples mixed with the 3% saline solution the sperms were killed. Then spectrophotometric concentration results were compared to the samples concentration in the Makler-chambers and searched for correlations between them. As a result, the following were obtained: all of the gold standard Makler-chambers concentration were compared to all spectrophotometric concentration and as a result for correlation coefficient we got 0,89. The highest correlation coefficient we got as a result was 0,70 diluted by semen extender on dilution 1:6, and the second highest coefficient was 0,69 on dilution 1:4. We got similar result when the diluent was 3% saline solution on dilution 1:6. There the correlation coefficient is 0,68.