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Home » Archive » 2018

TDK conference 2018

Antimicrobial susceptibility testing of bacteria isolated from urinary tract, respiratory tract, skin and ear infections of dogs in Hungary, in the period of 2015-2017
Kerekes Barbara - year 5
University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology
Supervisor: Dr. Ákos Jerzsele

Abstract:

Over the last few decades, antibiotic resistance among bacteria became widespread worldwide. As there are big differences among continents, countries or even in a single country, it is very important to acquire information regarding this issue in Hungary. The aim of our research is to explore the Hungarian situation about the main bacterial species involved in certain infections of dogs and to assess antibiotic resistance of these bacteria, as a thorough study on this has never been done before. Utilizing the results of our research we would like to give guidance for practitioners in the antibiotic therapy of companion animal infections.

Our co-operating laboratory was DUO-BAKT, collecting samples from the capital city and its surroundings. The data-collection represents an interval between 2015-2017 about the following specimen-types: urine, bronchoalveolar lavage (BAL), skin scraping, and auricular swab samples. Bacteria were cultured on different media; the antimicrobial susceptibility patterns were determined by disc-diffusion test. The data was systematized and stored with a computer software (Medbakter).

The bacteria isolated from urinary samples consisted mainly of Escherichia coli (41%), Staphylococcus pseudintermedius (18%), and Proteus mirabilis (15%). These bacteria showed an increased resistance to amoxicillin-clavulanate. The most frequently isolated bacterium from skin infections was S. pseudintermedius with a prevalence of 77%. The occurrence of all the other bacteria from these sample were under 4% for each. Amoxicillin-clavulanate and first generation cephalosporins are good first line options for treating pyoderma. From the auricual swab samples the three most frequently identified microbes were Malassezia pachydermatis (40%), S. pseudintermedius (23%), and Pseudomonas aeruginosa (15%). All the mentioned antibiotics for skin infections are effective for systemic treatment of otitis externa, but there is a great variability for topical treatment. Dominant isolates from BAL were Bordetella bronchiseptica (28%), P. aeruginosa (17%) and E. coli (14%). B. bronchiseptica were fully susceptible to amoxicillin-clavulanate and similarly, P. aeruginosa to polymyxin B. In the case of E. coli high resistance to frequently used antibiotics was found.

Because of the great variability of bacterial species causing infections and their susceptibility patterns it is highly recommended to send samples to microbiological laboratories for cultivation and susceptibility testing. Furthermore in case of acute, life-threatening infections, the local resistance patterns are essential to know for initiating antibiotic treatment.



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