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Home » Archive » 2022

TDK conference 2022

Comparison of dog sperm cryopreservation protocolls
Mészáros Eszter Luca - year 6
University of Veterinary Medicine Budapest, Department of Reproduction
Supervisors: Dr. Mónika Bacsa, Dr. Sándor Cseh

Abstract:

Deep freezing of canine semen is widely used for the long-term preservation of valuable breeding-male genetic material and transportation between countries. The composition and use of sperm freezing solutions have a major influence on the quality of the semen after thawing, which is best characterized by the progressive motility of spermatozoa.

In our study, we investigated the effectiveness of five commercially available sperm freezing solutions. The study aimed to determine which diluent gives the highest progressive motility values after thawing. The purpose of this research is to assist practicing veterinarians in the choice of sperm-freezing solutions from among those available on the market.

The studies were conducted in the Laboratory of Andrology and Assisted Reproduction at the University of Veterinary Medicine Budapest. The samples were taken from seven male Beagle dogs. The following diluents were tested in our study: CaniPro Freeze A&B (Minitube), Steridyl (Minitube), Steridyl + Equex pasta (Minitube), Triladyl (Minitube), CaniRep Uppsala Equex II (CaniRep HB, Uppsala). Fresh samples were tested with CASA software and then the sperm freezing solutions were applied as prescribed by the manufacturers. Each sample was cooled to 3-5 ℃ and incubated in nitrogen vapor then frozen at -196°C. The progressive motility of spermatozoa was checked in three phases: first fresh, after incubation, and after thawing.

The mean motility of fresh samples was 79.01 ± 7.32%. Fresh samples have not shown significant difference in the progressive motility. The results of incubated samples were lower than that of the fresh samples, only the Steridyl diluent showed a higher value. After thawing only CaniRep Uppsala Equex II showed no significant difference (P<0.05) in the progressive mean motility between fresh and thawed samples. Our tests showed that the progressive motility results after thawing can be better inferred from the results of incubated samples than from the data of fresh samples.

CaniRep Uppsala Equex II proved to be the best performing sperm freezing solution, the progressive motility of the thawed samples was 68.5 ± 20.59%.



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