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Home » Archive » 2022

TDK conference 2022

Antibiotic susceptibility testing of Pasteurella multocida isolates from rabbits
Graf Barnabás - year 4
University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology
Supervisor: Dr. Zoltán Somogyi

Abstract:

Pasteurella multocida is a Gram-negative bacterium responsible for the infection - commonly called pasteurellosis- of numerous wild and domestic animal species alike, such as rabbits, poultry, swine, cattle, buffalo, sheep, deer, camels and horses. P. multocida infection is also transmissible to humans via scratches and bites caused by animals. Clinical symptoms range from no clinical signs or mild chronic upper respitory inflammation to acute pneumonia or septicaemia. In the case of upper respitory infection, the leading clinical sign is rhinitis, while lower respitory pasteurellosis manifests predominantly as pneumonia with possible subcutaneous abscesses, especially in rabbits. The disease poses a great danger to rabbit stock around the world, while also leading to financial losses, to which a feasible solution is antibiotic therapy. In order to select the optimal antibiotic agent however, antibiotic susceptibility testing is required to maximise the well-being of animals and to minimize drug costs.

In our study we examined the antibiotic susceptibility of several Pasteurella multocida isolates from rabbits across Hungary. We used the microdilution method to determine the precise phenotypic susceptibility or Minimal Inhibitory Concentration (MIC) of 20 different antibiotic agents (amoxicillin, amoxicillin/clavulinic acid, ceftiofur, cefquinom, gentamicin, oxytetracycline, doxycycline, tylosin, tylmicosin, tylvalosin, tulathromycin, lincomycin, tiamulin, florfenicol, colistin, enrofloxacin, trimethroprim-sulfamethoxazole, vancomycin, imipenem) in 33 different rabbit P. multocida isolates. From the MIC value we further determined the MIC50 and the MIC90 values

In the case of amoxicillin, amoxicillin/clavulinic acid, ceftiofur and cefquinom we did not find especially high MIC values, whereas with neomycin and gentamicin we found one, and with oxytetracycline and doxycycline two isolates with an exceptionally high MIC value of 16-32 µg/ml. With the macrolide antibiotics, most of the isolates showed increased resistance towards tylosine and tylvalosin, while the majority of the isolates were susceptible to tylmicosin and tulathromycin. Almost all isolates were resistant to lincomycin, but susceptible to tiamulin and florfenicol. Colistin, enrofloxacin and trimethroprim-sulfamethoxazole proved to be effective. We found low MIC values in the case of imipenem and higher ones against vancomycin.



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