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TDK conference 2023Horváth Áron - year 4 University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology Supervisor: Ádám Kerek Antimicrobial resistance has become a key factor for the future of humanity, posing new challenges for animal and human health. My research focuses on the genus Enterococcus, which is of great public health importance, particularly in relation to multi-drug resistant and nosocomial infections. Previously, no comprehensive study has been conducted in Hungary to investigate the susceptibility of commensal Enterococcus strains of the poultry sector to antimicrobial agents of animal and public health significance at national and regional level. In our studies, minimum inhibitory concentration (MIC) values were determined, and the results were used to establish epidemiological thresholds (ECOFF) to differentiate wild-type and non-wild-type strains. Furthermore, our results were compared with human resistance data. The national ECOFF values for amoxicillin were found to be 8 µg/ml, which is in line with the results of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). For amoxicillin-clavulanic acid, however, a much higher value (32 µg/ml) was obtained than the 8 µg/ml value established by EUCAST. For doxycycline, a value of 2 µg/ml was obtained compared to the EUCAST value of 0.5 µg/ml. For florfenicol our results are very similar to the EUCAST breakpoint. For enrofloxacin, however, we obtained a much higher value (16 µg/ml) compared to the EUCAST value of 0.5 µg/ml. This is probably due to the overuse of fluoroquinolone active substances in the poultry sector. We also tested vancomycin, an active substance reserved for human health, and in this case our results were in line with EUCAST (4 µg/ml). At regional level, we observed significant differences, with several strains having ECOFF values more favourable than national and EUCAST values. Comparing the results with the human data it can be concluded that ampicillin resistance in human E. faecium strains is almost complete (98.5%) compared to 21.44% in animal samples. The level of human resistance to the life-saving drug vancomycin is also higher (43.6%) than that of animal samples (34.8%). Our results also justify the regional monitoring of resistance and the determination of ECOFF values, as the identification of the breakpoint between wild-type and non-wild-type strains can contribute to the selection of an effective dose, thus establishing the efficacy of treatment and minimising the chance of resistance development. List of lectures |