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Home » Archive » 2023

TDK conference 2023

Determination of the ECOFF (Epidemiological cut-off value) value of Escherichia coli bacterial strains isolated from domestic turkey farms
Gál Csaba - year 5
University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology
Supervisor: Dr. Ádám Kerek

Abstract:

One of the biggest challenges of the 21st century is the global spread of antimicrobial resistance. Regular assessments are needed to respond to the current situation in a timely manner. The comparison of the situation of resistance in humans with that of the commensals, clinical case samples and human resistance creates the One Health concept between animal and public health.

The poultry sector is one of the largest users of antibiotics, which also leads to widespread resistance. Our aim was to assess the situation of the turkey sector at national and regional level for Escherichia coli bacteria, antibiotics of animal and public health importance. To this end, we assessed the susceptibility of isolated commensal and clinical case strains by determining the minimum inhibitory concentration (MIC) value and compared our results with human resistance data. Finally, we determined epidemiological cut-off values (ECOFF) for the bimodal distribution data.

A total of 470 turkey-derived Escherichia coli strains were MIC determined for 15 different active substances, yielding MIC values of 7050. Based on the distribution of the data, ECOFF values were determined at national level for amoxicillin-clavulanic acid 128 µg/ml, ceftriaxone 0.25 µg/ml, imipenem 8 µg/ml, spectinomycin, doxycycline 1024 µg/ml, florfenicol 128 µg/ml and colistin 4 µg/ml. In a regional breakdown, ECOFF values for amoxicillin were successfully established for the regions of Közép-Magyarország and Nyugat-Dunántúl, with 32 µg/ml for the former and 16 µg/ml for the latter. Regionally, for enrofloxacin, the only region where a concentration of 0.5 µg/ml was acceptable was the Dél-Alföld region. For the active substance colistin, a value of 1 µg/ml was obtained in most regions, with the only higher value (4 µg/ml) being in the Nyugat-Dunántúl region. For amoxicillin, both the commensal and clinical strains showed the same level of resistance (73%), and for the corresponding ampicillin drug, the human strains showed very similar levels of resistance (52%). For the other drugs, the resistance situation is much less favourable in the veterinary line.

Our results support the rationale for defining ECOFF values that can contribute to the timely and appropriate management of antimicrobial resistance based on regional antibiotic use patterns. Regular surveys and comparisons with human data create the concept of One Health.



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