|
||||
Home
» Archive
» 2023
TDK conference 2023Román István László - year 5 University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology Supervisor: Dr. Ádám Kerek Antimicrobial resistance has emerged as the leading global problem of the 21st century. The poultry sector, a leading user of antibiotics, is of particular importance in animal health. The link between animal and public health observed in nosocomial infections suggests that one in five cases can be traced back to the use of antibiotics in veterinary medicine with insufficient care. In our study, we aimed to determine the minimum inhibitory concentration (MIC) values of commensal Escherichia coli strains isolated from oral cavity and cloacal swab samples from large domestic chicken colonies representative of seven regions of Hungary for the active substances of therapeutic and public health importance. We then used our results to establish so-called Epidemiological cut-off values (ECOFF) at national and regional level. A total of 411 strains of domestic fowl origin were tested for 15 different active substances, using 6150 MIC values to successfully determine the ECOFF of amoxicillin-clavulanic acid, imipenem, neomycin, spectinomycin, doxycycline, florfenicol, tiamulin, enrofloxacin and colistin at national level. The resistance values of the commensal strains were compared with the results of strains collected from clinical cases (n=133) and with data from human health (n=58 168). Our results showed very high ECOFF values for amoxicillin-clavulanic acid (128 µg/ml), imipenem (16 µg/ml), neomycin (2024 µg/ml), spectinomycin (512 µg/ml), doxycycline (512 µg/ml), florfenicol (128 µg/ml), tiamulin (1024 µg/ml) and enrofloxacin (128 µg/ml) at the national level. Only for colistin (4 µg/ml) were the results very similar to those of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2 µg/ml). The commensal strains showed very similar resistance profiles compared to the animal clinical samples; however, for the human data, only amoxicillin showed similar results, with the human samples being more sensitive to the other drugs. In our study, when using the defined ECOFF values as breakpoints, the bacterial population tested showed a much higher sensitivity to each of the active substances. Our results support the justification for ECOFF testing, as currently there is a breakpoint value for Escherichia coli species for domestic fowl established by the Clinical and Laboratory Standards Institute (CLSI) for enrofloxacin only. List of lectures |