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TDK conference 2023Kovács Fruzsina - year 6 University of Veterinary Medicine Budapest, Department of Microbiology and Infectious Diseases Supervisor: Dr. Tóth Gergely Diseases caused by Mannheimia haemolytica and Bibersteinia trehalosi are common, with significant economic losses. With the widespread use of antibiotics to control these diseases, the rate of antibiotic resistance is increasing all over the world. Due to ineffective treatments, there is a constant risk that we will not be able to control diseases. In animal husbandry, the economy of production is decreasing, and the biosafety of animal products can be guaranteed less and less. Therefore, we have to moderate the use of antibiotics, promote prudent antibiotic use. The first step in this case to assess the existing resistance conditions from time to time and compose recommendations referring to the appropriate use of drugs. The aim of our study was to determine antibiotic sensitivity of Mannheimia haemolytica and Bibersteinia trehalosi strains isolated from respiratory diseases in Hungarian cattle using two different methods. We obtained our samples from a total of 39 farms in Hungary (35 M. haemolytica, 10 Bibersteinia trehalosi, 1 M. glucosida). Strains from different serotype were selected from one place of origin. The antibiotic sensitivity test was performed with 11 antibiotics (amoxicillin, ceftiofur, enrofloxacin, florfenicol, gentamicin, oxytetracycline, sulfamethoxazole-trimethoprim, penicillin, tiamulin, tilmicosin and tulathromycin), which represent 10 antibiotic classes. Disk diffusion and broth dilution micro methods were also used, and their results were compared. In our study, during determining the MIC value, most bacteria showed sensitivity to oxytetracycline, enrofloxacin, amoxicillin, florfenicol, gentamicin, sulfamethoxazole-trimethoprim and ceftiofur. In the case of oxytetracycline, florfenicol, tulathromycin, the MIC values obtained the disc diffusion test were highly correlated. However, the results obtained in the case of enrofloxacin, amoxicillin, gentamicin, sulfamethoxazole-trimethroprim, tiamulin, tilmicosin and penicillin were divergent. In the case of ceftiofur, resistant bacterial strains were found only using the broth dilution micro method, but not during the disc diffusion test. Similar to the international studies, florfenicol proved to be the safest antibiotic, we observed the least resistant strains against it. On the other hand, a high degree of resistance was observed in the case of tiamulin and penicillin-G. List of lectures |