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Home » Archive » 2023

TDK conference 2023

Protective effect of probiotic bacteria on bovine endometrial cells, in vitro
Sequeira Lindsay Mary - year 5
University of Veterinary Medicine Budapest, Department of Pharmacology and Toxicology
Supervisors: Palócz Orsolya, Csikó György

Abstract:

Lactobacillus is a well-known genus of bacteria that is isolated from various food products and hosts. They are normal residents of the gut and vaginal epithelium in humans and animals. Although, this genus is most known as a safe and beneficial probiotic, it also exerts anti-pathogenic, anti-fungal and antioxidant effects.

Dairy cows are incredibly susceptible to uterine infection postpartum. Usually, metritis is the consequence of the contamination by bacterial agents like Trueperella pyogenes. Metritis could lead to delayed involution, issues in folliculogenesis and ovarian function, and infertility causing production loss and increased culling rate. Antibiotics are usually used to treat this disease condition, which are somewhat effective, but their usage has limitations. Furthermore, with the steady rise in antibiotic resistance and its implications, research is being conducted for alternative treatment options, such as probiotics.

In this study we aim to evaluate the probiotic potential of the mixture of four lactobacilli strains. Primary bovine endometrial epithelial cells (BEnEpC) were collected from non-pregnant, healthy cows and were cultured in 6-well culture plates. BEnEpC cultures were co-cultured with T. pyogenes alone or together with the four lactobacilli. Cells cultured with the same medium but without bacteria served as controls. The changes in levels of pro-inflammatory mediators like IL-3, IL-6, IL-8, and TNF were also investigated.

The BEnEpC culture with the lactobacilli and T. pyogenes showed significant downregulation of the IL-3 and TNF expression in comparison to the T. pyogenes culture. However, there was significant upregulation seen in IL-8 expression in all cultures. IL-6 expression was slightly downregulated in the lactobacilli culture-treated endometrial cells, and slightly upregulated in the T. pyogenes and lactobacilli mixed culture as compared to T. pyogenes alone.

In conclusion, the results exhibit some probiotic potential of lactobacilli in the attenuation of metritis in cows. However, it must be considered that during the research phase, in vitro studies provide a good first approach, but the results obtained must also be confirmed by live animal studies.



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