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Home » Archive » 2014

TDK conference 2014

Anti-inflammatory activity of flavonoids on swine intestinal epithelial cells
Balázs Anna - year 5
SZIU Faculty of Veterinary Science, Department of Pharmacology and Toxicology
Supervisor: Dr. Orsolya Farkas

Abstract:

In the past few years there have been a greater interest in flavonoids, due to their antioxidant activity. Furthermore, several properties have been ascribed to flavonoids such as anti-cancer, antiallergic and anti-inflammatory effect. For these reasons is feasible to use them both in human and veterinary medicine, for example as a promising new class of anti-inflammatory agents with less side-effects than the non-steroid anti-inflammatory drugs (NSAIDs). Moreover, the antioxidant activity and the fact that flavonoids are able to moderate the inflammatory response enable to use them as growth promoter in livestock production. Our aim is to investigate the anti-inflammatory effect of apigenin and its trimethylated analogue, trimethoxyapigenin on intestinal epithelial cells.

In the experiments IPEC-J2 non-transformed swine intestinal epithelial cells were used. The cells were seeded onto collagen-coated polyester membrane inserts. In the growth period the cell monolayer integrity was controlled by transepithelial electrical resistance measurement. Inflammation was evoked by bacterial lipopolysaccharide (LPS). The levels of IL-6, IL-8, TNF-α and COX-2 gene-expression were measured by real-time PCR. Both the apigenin and the trimethoxyapigenin concentration was 25 µM.

After 10 µg/ml LPS treatment the relative gene expression of all the assayed proinflammatory cytokines were significantly increased. The gene expression of IL-6 was significantly decreased by apigenin. Trimethoxy-apigenin did not influence the IL-6 mRNA level. Both apigenin and trimethoxyapigenin reduced the level of IL-8 expression significantly. Treatment with trimethoxyapigenin lowered the gene expression of TNF-α, while apigenin did not show that activity. Apigenin as well as trimethoxyapigenin caused significant reduction in the level of COX-2 gene expression, but the methylated analogue proved to be more effective than the unmethylated one.

In conclusion, our results suggest that both apigenin and trimethoxyapigenin can modulate the LPS activated intestinal inflammatory response. Further research should deal with understanding the exact mechanism that lie behind their anti-inflammatory properties and unravelling the differences in the bioactivity of the methylated and unmethylated analogues. Nevertheless, there is specified need of in vivo studies.



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